BIOCHIPS

Acoustic micromixer for enhancement of DNA biochip systems

[+] Author Affiliations
Robin Hui Liu, Ralf Lenigk, Piotr Grodzinski

Motorola Labs, Microfluidics Laboratory, 7700 South River Parkway ML34, Tempe, Arizona 85284

J. Micro/Nanolith. MEMS MOEMS. 2(3), 178-184 (Jul 01, 2003). doi:10.1117/1.1582467
History: Received Sep. 19, 2002; Revised Mar. 5, 2003; Accepted Mar. 27, 2003; Online July 07, 2003
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Microfluidics is emerging as one of the fastest growing segments of micro-electro-mechanical system (MEMS) technologies due to its potential applications in biotechnology, chemical microreactors, and drug discovery. Micromixing is one of the most challenging problems in microfluidic systems, since it is a diffusion-limited process and can be very inefficient. A micromixing device based on an acoustic microstreaming principle is developed to enhance micromixing. The micromixer uses air bubbles as actuators that can be set into vibration by a sound field. The vibration of the air bubbles generates steady circulatory flows, resulting in global convection flows and thus rapid mixing. The time to fully mix dyed solutions in a 50-μL shallow chamber using acoustic microstreaming is significantly reduced from hours (a pure diffusion-based mixing) to 6 s. We demonstrate the use of this micromixer to enhance the performance of conventional DNA microarray biochips that often suffer from lengthy hybridization and poor signal uniformity due to a diffusion-limited hybridization process. Experiments showed that the acoustic micromixer results in five-fold hybridization signal enhancement with significantly improved signal uniformity, as compared to conventional diffusion-based biochips. Acoustic microstreaming has many advantages over most existing micromixing techniques, including a simple apparatus, ease of implementation, low power consumption (∼ 2 mW), and low cost. © 2003 Society of Photo-Optical Instrumentation Engineers.

© 2003 Society of Photo-Optical Instrumentation Engineers

Citation

Robin Hui Liu ; Ralf Lenigk and Piotr Grodzinski
"Acoustic micromixer for enhancement of DNA biochip systems", J. Micro/Nanolith. MEMS MOEMS. 2(3), 178-184 (Jul 01, 2003). ; http://dx.doi.org/10.1117/1.1582467


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