Special Section on Theory and Practice of MEMS/NEMS/MOEMS, RF MEMS, and BioMEMS

Design and numerical analysis of a joule-heating-induced continuous-flow polymerase chain reaction microchip

[+] Author Affiliations
Qiaole Zhao

Southeast University, Key Laboratory of MEMS of the Ministry of Education, Nanjing, China

Qing-An Huang

Southeast University, Key Laboratory of MEMS of the Ministry of Education, Nanjing, China

Yu-Cheng Lin

National Cheng Kung University, Department of Engineering Science, and Center for Micro/Nano Science and Technology, 1 University Road, Tainan 701, Taiwan

J. Micro/Nanolith. MEMS MOEMS. 8(2), 021102 (April 02, 2009). doi:10.1117/1.3091940
History: Received August 14, 2008; Revised November 07, 2008; Accepted November 11, 2008; Published April 02, 2009
Text Size: A A A

We reports a detailed study on a novel design of a Joule-heating-induced polymerase chain reaction (PCR) microchip directly heated by applying electric current to the ends of the geometric varied microchannel, which is simulated together with the factors acting on the temperature distribution. Treating the continuous flowing fluid in the channel as the resistance, different channel shapes would lead to the different resistances, which generates various heat when the fixed current is applied to the ends of channel. Based on the Ohm’s law and the Joule-Thomson effect, three required sequential temperature zones are obtained. The temperature cycling is achieved and the factors that contribute to the temperature distribution are simulated and analyzed, such as the environment temperature, the heat transfer coefficient, the current density, the fluid velocity, and the channel shape. The design of the Joule-heating-induced PCR microchip sheds light on the future novel application of continuous-flow PCR. By applying certain conditions, thermal cycles that meet PCR requirements can be achieved.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Qiaole Zhao ; Qing-An Huang and Yu-Cheng Lin
"Design and numerical analysis of a joule-heating-induced continuous-flow polymerase chain reaction microchip", J. Micro/Nanolith. MEMS MOEMS. 8(2), 021102 (April 02, 2009). ; http://dx.doi.org/10.1117/1.3091940


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.