Articles

Obstacles to the production of protein microarray cassettes

[+] Author Affiliations
Jean Montagu

Decision BioMarkers Inc., 150 Bear Hill Road, Waltham, Massachusetts 02451

Herman DeWeerd

Decision BioMarkers Inc., 150 Bear Hill Road, Waltham, Massachusetts 02451

Nathan Tyburczy

Decision BioMarkers Inc., 150 Bear Hill Road, Waltham, Massachusetts 02451

Natalia Rodionova

Decision BioMarkers Inc., 150 Bear Hill Road, Waltham, Massachusetts 02451

Peter Maimonis

Decision BioMarkers Inc., 150 Bear Hill Road, Waltham, Massachusetts 02451

J. Micro/Nanolith. MEMS MOEMS. 8(3), 033013 (July 16, 2009). doi:10.1117/1.3158071
History: Received March 23, 2009; Revised April 24, 2009; Accepted May 04, 2009; Published July 16, 2009
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We examine the necessary technologies to be mastered in order to build a practical microarray-based immunoassay cassette and its processing station for protein analysis. The interdependence of surface chemistry, dye stability, and imaging are outlined, showing why a treated 100-nm film of nitrocellulose adhered by an intervening layer to glass offers an efficacious surface for immobilizing an array of protein probes. The properties of a storage surface to support in desiccated form, fluidize and transport additional reagents are outlined, and a practical solution proposed. Wet and dry imaging are compared. The steps and functions expected for an assay platform comprising a processing station and biochip cassette are identified. The performance of a successful benchtop automated multiplex immunoassay system is briefly described.

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© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Jean Montagu ; Herman DeWeerd ; Nathan Tyburczy ; Natalia Rodionova and Peter Maimonis
"Obstacles to the production of protein microarray cassettes", J. Micro/Nanolith. MEMS MOEMS. 8(3), 033013 (July 16, 2009). ; http://dx.doi.org/10.1117/1.3158071


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