Obstacles to the production of protein microarray cassettes

Jean I. Montagu; Herman DeWeerd; Nathan Tyburczy; Natalia Rodionova; Peter Maimonis

doi:10.1117/1.3158071

1 July 2009 Obstacles to the production of protein microarray cassettes

Jean I. Montagu, Herman DeWeerd, Nathan Tyburczy, Natalia Rodionova, Peter Maimonis

Journal of Micro/Nanolithography, MEMS, and MOEMS, Vol. 8, Issue 3, 033013 (July 2009). https://doi.org/10.1117/1.3158071

Abstract

We examine the necessary technologies to be mastered in order to build a practical microarray-based immunoassay cassette and its processing station for protein analysis. The interdependence of surface chemistry, dye stability, and imaging are outlined, showing why a treated 100-nm film of nitrocellulose adhered by an intervening layer to glass offers an efficacious surface for immobilizing an array of protein probes. The properties of a storage surface to support in desiccated form, fluidize and transport additional reagents are outlined, and a practical solution proposed. Wet and dry imaging are compared. The steps and functions expected for an assay platform comprising a processing station and biochip cassette are identified. The performance of a successful benchtop automated multiplex immunoassay system is briefly described.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Jean I. Montagu, Herman DeWeerd, Nathan Tyburczy, Natalia Rodionova, and Peter Maimonis "Obstacles to the production of protein microarray cassettes," Journal of Micro/Nanolithography, MEMS, and MOEMS 8(3), 033013 (1 July 2009). https://doi.org/10.1117/1.3158071

Published: 1 July 2009

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available