Articles

Mechanical characterization and insertion performance of hollow microneedle array for cell surgery

[+] Author Affiliations
Takahiro Kawashima

Toyohashi University of Technology, Department of Production Systems Engineering, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi 441-8580, Japan

Takahiro Sakai

Toyohashi University of Technology, Department of Production Systems Engineering, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi 441-8580, Japan

Norihisa Kato

Toyohashi University of Technology, Department of Production Systems Engineering, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi 441-8580, Japan

Takayuki Shibata

Toyohashi University of Technology, Department of Production Systems Engineering, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi 441-8580, Japan

Mitsuyoshi Nomura

Toyohashi University of Technology, Department of Production Systems Engineering, 1-1 Hibarigaoka, Tempaku-cho, Toyohashi 441-8580, Japan

Takashi Mineta

Hirosaki University, Faculty of Science and Technology, Department of Intelligent Machines and System Engineering, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan

Eiji Makino

Hirosaki University, Faculty of Science and Technology, Department of Intelligent Machines and System Engineering, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan

J. Micro/Nanolith. MEMS MOEMS. 8(3), 033014 (September 15, 2009). doi:10.1117/1.3206971
History: Received August 15, 2008; Revised May 30, 2009; Accepted July 07, 2009; Published September 15, 2009
Text Size: A A A

In order to implement cell surgery on a chip-based system, we have been developing microneedle arrays capable of introducing desired biomolecules (nucleic acids, proteins, etc.) into living cells and the parallel extracting biomolecules expressed in the cells. An array of hollow silicon dioxide (SiO2) microneedles with a sharp tip radius of less than 0.5μm was successfully fabricated by using a micromachining technique. In order to investigate the mechanical stability of fabricated microneedle arrays, insertion tests with a gelatin as an artificial cell were performed. The results indicated that the microneedles are expected to be sufficiently stiff to insert into living cells without fracture. In addition, bending behavior was characterized by both finite element method (FEM) analysis and experimental fracture test. Needle insertion performance into gelatin was also evaluated. The displacement required for needle insertion increased linearly with an increase in surface area at the needle tip, resulting in the relative value of estimated insertion stresses being approximately constant. Moreover, the results showed that the mechanical oscillation with an amplitude of 0.6μm was effective and that increasing oscillation frequency decreased remarkably the displacement probably due to an increase in the viscous resistance of a viscoelastic material.

Figures in this Article
© 2009 Society of Photo-Optical Instrumentation Engineers

Citation

Takahiro Kawashima ; Takahiro Sakai ; Norihisa Kato ; Takayuki Shibata ; Mitsuyoshi Nomura, et al.
"Mechanical characterization and insertion performance of hollow microneedle array for cell surgery", J. Micro/Nanolith. MEMS MOEMS. 8(3), 033014 (September 15, 2009). ; http://dx.doi.org/10.1117/1.3206971


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.