Articles

Effects of a glutaraldehyde cross-linking layer on a quartz crystal microbalance–based α-fetoprotein biosensor with cystamine self-assembly monolayer

[+] Author Affiliations
I-Yu Huang

National Sun Yat-Sen University, Department of Electrical Engineering, Kaohsiung 80424, Taiwan

Chang-Yu Lin

National Sun Yat-Sen University, Department of Electrical Engineering, Kaohsiung 80424, Taiwan

En-Chang Wu

National Sun Yat-Sen University, Department of Electrical Engineering, Kaohsiung 80424, Taiwan

J. Micro/Nanolith. MEMS MOEMS. 9(2), 023008 (April 22, 2010). doi:10.1117/1.3415495
History: Received July 23, 2009; Revised March 06, 2010; Accepted March 15, 2010; Published April 22, 2010; Online April 22, 2010
Text Size: A A A

To identify the extreme low concentration of alpha-fetoprotein (AFP) antigen in human serum for early detection of hepatocellular carcinoma, we aim to develop a high-sensitivity and low-cost AFP biosensor using quartz crystal microbalance (QCM) and cystamine self-assembly monolayer (SAM) technologies. In this study, the surface topographies of concentrations (0.1 and 1.0mgmL) of AFP antibody with and without 1.25% glutaraldehyde cross-linking layer will be analyzed by an atomic force microscope system to investigate the effects of the glutaraldehyde layer on the sensing characteristics of the QCM-based AFP biosensor. According to our experimental results, the mass sensitivity was improved almost doubly (from 0.07to0.146HzmLmg1) as the glutaraldehyde layer was added between the 20-mM cystamine SAM and the low-concentration (0.1mgmL) AFP antibody. Either with or without the glutaraldehyde layer, higher mass sensitivity (0.163to0.335HzmLmg1) was obtained as the AFP antibody concentration increased to 1.0mgmL. However, a large AFP antibody requirement will increase fabrication cost and limit disposable application. We also demonstrated that high sensing linearities (95.67 to 99.6%) of the QCM-AFP biosensors can be achieved without being obviously affected by the glutaraldehyde layer and AFP antibody concentration.

Figures in this Article
© 2010 Society of Photo-Optical Instrumentation Engineers

Citation

I-Yu Huang ; Chang-Yu Lin and En-Chang Wu
"Effects of a glutaraldehyde cross-linking layer on a quartz crystal microbalance–based α-fetoprotein biosensor with cystamine self-assembly monolayer", J. Micro/Nanolith. MEMS MOEMS. 9(2), 023008 (April 22, 2010). ; http://dx.doi.org/10.1117/1.3415495


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.