Polymer-based microfluidic devices can be fabricated in several ways, such as laser ablation,8–10 hot embossing,11–13 injection molding,14–16 and deep-UV patterning.17–19 Polymers, such as polymethylmethacrylate (PMMA), are highly transparent and have very low autoﬂuorescence over a wide spectral range,20 making it a well used material in microfluidics. The PMMA can be patterned in all the above ways; however, hot embossing and injection molding are high-volume methods and have high-tooling costs, making their applicability to a research environment low. Direct laser ablation is usually used for rapid prototyping, which produces rough channels surfaces and make the observation difficult. The deep-UV (254 nm) patterning can be performed in hours and produces smooth channel surfaces for easy observation. The PMMA absorbs radiation during the deep-UV exposure, which causes chain scissions and increased solubility. Exposed PMMA can be developed using a mixture of isopropanol alcohol (IPA) and water; this developer could be used not only for the microscale structure,21 but also for the nanoscale patterning of PMMA.22,23 In our previous work, instead of using the traditional mask during deep-UV patterning, we transferred printed wax layers to act as a deep-UV mask;19 however, edge quality was low. In comparison, the method described in this study allows for the patterning of both the channels, with improved edge quality, and electrode layers for microfluidic devices using wax-covered plastic paper. This simple technical innovation will enable the lab-on-chip community to use equipment they likely already have to rapidly prototype higher quality channels with integrated electrodes.