Regular Articles

Development of microarray device for functional evaluation of PC12D cell axonal extension ability

[+] Author Affiliations
Eiji Nakamachi

Doshisha University, Department of Biomedical Engineering, 1-3 Miyakodani, Tatara, Kyotanabe City, Kyoto 610-0394, Japan

Junpei Yanagimoto

Doshisha University, Department of Biomedical Engineering, 1-3 Miyakodani, Tatara, Kyotanabe City, Kyoto 610-0394, Japan

Shinya Murakami

Doshisha University, Department of Biomedical Engineering, 1-3 Miyakodani, Tatara, Kyotanabe City, Kyoto 610-0394, Japan

Yusuke Morita

Doshisha University, Department of Biomedical Engineering, 1-3 Miyakodani, Tatara, Kyotanabe City, Kyoto 610-0394, Japan

J. Micro/Nanolith. MEMS MOEMS. 13(2), 023013 (Jun 27, 2014). doi:10.1117/1.JMM.13.2.023013
History: Received February 16, 2014; Revised May 24, 2014; Accepted May 30, 2014
Text Size: A A A

Abstract.  In this study, we developed a microarray bio-MEMS device that could trap PC12D (rat pheochromocytoma cells) cells to examine the intercellular interaction effect on the cell activation and the axonal extension ability. This is needed to assign particular patterns of PC12D cells to establish a cell functional evaluation technique. This experimental observation-based technique can be used for design of the cell sheet and scaffold for peripheral and central nerve regeneration. We have fabricated a micropillar-array bio-MEMS device, whose diameter was approximately 10 μm, by using thick photoresist SU-8 on the glass slide substrate. A maximum trapped PC12D cell ratio, 48.5%, was achieved. Through experimental observation of patterned PC12D “bi-cells” activation, we obtained the following results. Most of the PC12D “bi-cells” which had distances between 40 and 100 μm were connected after 24 h with a high probability. On the other hand, “bi-cells” which had distances between 110 and 200 μm were not connected. In addition, we measured axonal extension velocities in cases where the intercellular distance was between 40 and 100 μm. A maximum axonal extension velocity, 86.4μm/h, was obtained at the intercellular distance of 40 μm.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Eiji Nakamachi ; Junpei Yanagimoto ; Shinya Murakami and Yusuke Morita
"Development of microarray device for functional evaluation of PC12D cell axonal extension ability", J. Micro/Nanolith. MEMS MOEMS. 13(2), 023013 (Jun 27, 2014). ; http://dx.doi.org/10.1117/1.JMM.13.2.023013


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.