Development of microarray device for functional evaluation of PC12D cell axonal extension ability

Eiji Nakamachi; Junpei Yanagimoto; Shinya Murakami; Yusuke Morita

doi:10.1117/1.JMM.13.2.023013

27 June 2014 Development of microarray device for functional evaluation of PC12D cell axonal extension ability

Eiji Nakamachi, Junpei Yanagimoto, Shinya Murakami, Yusuke Morita

Journal of Micro/Nanolithography, MEMS, and MOEMS, Vol. 13, Issue 2, 023013 (June 2014). https://doi.org/10.1117/1.JMM.13.2.023013

Abstract

In this study, we developed a microarray bio-MEMS device that could trap PC12D (rat pheochromocytoma cells) cells to examine the intercellular interaction effect on the cell activation and the axonal extension ability. This is needed to assign particular patterns of PC12D cells to establish a cell functional evaluation technique. This experimental observation-based technique can be used for design of the cell sheet and scaffold for peripheral and central nerve regeneration. We have fabricated a micropillar-array bio-MEMS device, whose diameter was approximately 10 μm, by using thick photoresist SU-8 on the glass slide substrate. A maximum trapped PC12D cell ratio, 48.5%, was achieved. Through experimental observation of patterned PC12D “bi-cells” activation, we obtained the following results. Most of the PC12D “bi-cells” which had distances between 40 and 100 μm were connected after 24 h with a high probability. On the other hand, “bi-cells” which had distances between 110 and 200 μm were not connected. In addition, we measured axonal extension velocities in cases where the intercellular distance was between 40 and 100 μm. A maximum axonal extension velocity, 86.4 μm/h, was obtained at the intercellular distance of 40 μm.

Citation Download Citation

Eiji Nakamachi, Junpei Yanagimoto, Shinya Murakami, and Yusuke Morita "Development of microarray device for functional evaluation of PC12D cell axonal extension ability," Journal of Micro/Nanolithography, MEMS, and MOEMS 13(2), 023013 (27 June 2014). https://doi.org/10.1117/1.JMM.13.2.023013

Published: 27 June 2014

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available