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Total internal reflection–based module for fluorescence and absorbance detection

[+] Author Affiliations
Tom Verschooten

Vrije Universiteit Brussel, Department of Applied Physics and Photonics, Brussels Photonics Team, Pleinlaan 2, 1050 Brussels, Belgium

Heidi Ottevaere

Vrije Universiteit Brussel, Department of Applied Physics and Photonics, Brussels Photonics Team, Pleinlaan 2, 1050 Brussels, Belgium

Michael Vervaeke

Vrije Universiteit Brussel, Department of Applied Physics and Photonics, Brussels Photonics Team, Pleinlaan 2, 1050 Brussels, Belgium

Jürgen Van Erps

Vrije Universiteit Brussel, Department of Applied Physics and Photonics, Brussels Photonics Team, Pleinlaan 2, 1050 Brussels, Belgium

Hugo Thienpont

Vrije Universiteit Brussel, Department of Applied Physics and Photonics, Brussels Photonics Team, Pleinlaan 2, 1050 Brussels, Belgium

J. Micro/Nanolith. MEMS MOEMS. 13(3), 033001 (Jul 03, 2014). doi:10.1117/1.JMM.13.3.033001
History: Received April 11, 2014; Revised May 30, 2014; Accepted June 2, 2014
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Abstract.  We present a miniaturized polymer-based micro-optical detection unit for ultraviolet and visible laser-induced fluorescence (LIF) and absorbance (ABS) analysis with an interaction length of 3 mm. We use nonsequential optical ray tracing simulations to model the system and to optimize its performance with respect to optical efficiency and system complexity. The design features a compact optical system combining total internal reflection (TIR) mirrors and refractive optics. The detection module is prototyped with deep proton writing in 2-mm-thick polymethylmethacrylate and quantitatively characterized using a three-dimensional coordinate measurement machine. We demonstrate the proof-of-concept of this TIR mirror–based module for coumarin 480 obtaining limits of detection of 50 pM and 500 nM for LIF and ABS, respectively.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Tom Verschooten ; Heidi Ottevaere ; Michael Vervaeke ; Jürgen Van Erps and Hugo Thienpont
"Total internal reflection–based module for fluorescence and absorbance detection", J. Micro/Nanolith. MEMS MOEMS. 13(3), 033001 (Jul 03, 2014). ; http://dx.doi.org/10.1117/1.JMM.13.3.033001


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