Microfabrication

One-step fabrication of three-dimensional polydimethylsiloxane peel-off microwell array for cell trapping

[+] Author Affiliations
Ning Xue

University of Texas at Dallas, Department of Electrical Engineering, 800 West Campbell Road, Richardson, Texas 75080, United States

Institute of Microelectronics, 11 Science Park Road, Singapore 117685, Singapore

Leonidas Bleris

University of Texas at Dallas, Department of Electrical Engineering, 800 West Campbell Road, Richardson, Texas 75080, United States

University of Texas at Dallas, Department of Bioengineering Engineering, 800 West Campbell Road, Richardson, Texas 75080, United States

Jeong-Bong (JB) Lee

University of Texas at Dallas, Department of Electrical Engineering, 800 West Campbell Road, Richardson, Texas 75080, United States

J. Micro/Nanolith. MEMS MOEMS. 14(1), 014503 (Feb 19, 2015). doi:10.1117/1.JMM.14.1.014503
History: Received November 9, 2014; Accepted January 30, 2015
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Abstract.  A three-dimensional polydimethylsiloxane (PDMS)-based microarray was one-step fabricated by releasing from an SU-8 mold for cell trapping. A human embryonic kidney-293 cell line was cultured on the PDMS microarray. By taking advantage of the chemical nature difference between PDMS surface released from oxidized silicon and released from SU-8 substrate, cells can only grow and be attached inside the PDMS microarray after 2 days of cell culture. To investigate the substantial nature of the cell adhesion and repellency on the PDMS substrate, atomic force microscopy, contact angle, and x-ray photoelectron spectroscopy methods have been carried out to analyze the physical and chemical properties of the PDMS material surfaces under different surface treatment circumstances.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Ning Xue ; Leonidas Bleris and Jeong-Bong (JB) Lee
"One-step fabrication of three-dimensional polydimethylsiloxane peel-off microwell array for cell trapping", J. Micro/Nanolith. MEMS MOEMS. 14(1), 014503 (Feb 19, 2015). ; http://dx.doi.org/10.1117/1.JMM.14.1.014503


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