Microfabrication

Low-temperature bonding process for the fabrication of hybrid glass–membrane organ-on-a-chip devices

[+] Author Affiliations
Kyall J. Pocock, Craig Priest, Clive A. Prestidge, Benjamin Thierry

University of South Australia, Future Industries Institute, Mawson Lakes Boulevard, Adelaide 5095, Australia

Xiaofang Gao, Kazuma Mawatari, Takehiko Kitamori

University of Tokyo, School of Engineering, Department of Applied Chemistry, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan

Chenxi Wang

Harbin Institute of Technology, State Key Laboratory of Advanced Welding and Joining, 92 West Dazhi Street, Nan Gang District, Harbin 150001, China

J. Micro/Nanolith. MEMS MOEMS. 15(4), 044502 (Dec 07, 2016). doi:10.1117/1.JMM.15.4.044502
History: Received June 27, 2016; Accepted November 8, 2016
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Abstract.  The integration of microfluidics with living biological systems has paved the way to the exciting concept of “organs-on-a-chip,” which aims at the development of advanced in vitro models that replicate the key features of human organs. Glass-based devices have long been utilized in the field of microfluidics but the integration of alternative functional elements within multilayered glass microdevices, such as polymeric membranes, remains a challenge. To this end, we have extended a previously reported approach for the low-temperature bonding of glass devices that enables the integration of a functional polycarbonate porous membrane. The process was initially developed and optimized on specialty low-temperature bonding equipment (μTAS2001, Bondtech, Japan) and subsequently adapted to more widely accessible hot embosser units (EVG520HE Hot Embosser, EVG, Austria). The key aspect of this method is the use of low temperatures compatible with polymeric membranes. Compared to borosilicate glass bonding (650°C) and quartz/fused silica bonding (1050°C) processes, this method maintains the integrity and functionality of the membrane (Tg 150°C for polycarbonate). Leak tests performed showed no damage or loss of integrity of the membrane for up to 150 h, indicating sufficient bond strength for long-term cell culture. A feasibility study confirmed the growth of dense and functional monolayers of Caco-2 cells within 5 days.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Kyall J. Pocock ; Xiaofang Gao ; Chenxi Wang ; Craig Priest ; Clive A. Prestidge, et al.
"Low-temperature bonding process for the fabrication of hybrid glass–membrane organ-on-a-chip devices", J. Micro/Nanolith. MEMS MOEMS. 15(4), 044502 (Dec 07, 2016). ; http://dx.doi.org/10.1117/1.JMM.15.4.044502


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