Chemiluminescence generation and detection in a capillary-driven microfluidic chip

Charlotte Ramon; Yuksel Temiz; Emmanuel Delamarche

doi:10.1117/12.2250765

28 February 2017 Chemiluminescence generation and detection in a capillary-driven microfluidic chip

Charlotte Ramon, Yuksel Temiz, Emmanuel Delamarche

Proceedings Volume 10061, Microfluidics, BioMEMS, and Medical Microsystems XV; 100610O (2017) https://doi.org/10.1117/12.2250765
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The use of microfluidic technology represents a strong opportunity for providing sensitive, low-cost and rapid diagnosis at the point-of-care and such a technology might therefore support better, faster and more efficient diagnosis and treatment of patients at home and in healthcare settings both in developed and developing countries. In this work, we consider luminescence-based assays as an alternative to well-established fluorescence-based systems because luminescence does not require a light source or expensive optical components and is therefore a promising detection method for point-of-care applications. Here, we show a proof-of-concept of chemiluminescence (CL) generation and detection in a capillary-driven microfluidic chip for potential immunoassay applications. We employed a commercial acridan-based reaction, which is catalyzed by horseradish peroxidase (HRP). We investigated CL generation under flow conditions using a simplified immunoassay model where HRP is used instead of the complete sandwich immunocomplex. First, CL signals were generated in a capillary microfluidic chip by immobilizing HRP on a polydimethylsiloxane (PDMS) sealing layer using stencil deposition and flowing CL substrate through the hydrophilic channels. CL signals were detected using a compact (only 5×5×2.5 cm³) and custom-designed scanner, which was assembled for less than $30 and comprised a 128×1 photodiode array, a mini stepper motor, an Arduino microcontroller, and a 3D-printed housing. In addition, microfluidic chips having specific 30-μm-deep structures were fabricated and used to immobilize ensembles of 4.50 μm beads functionalized with HRP so as to generate high CL signals from capillary-driven chips.

Citation Download Citation

Charlotte Ramon, Yuksel Temiz, and Emmanuel Delamarche "Chemiluminescence generation and detection in a capillary-driven microfluidic chip", Proc. SPIE 10061, Microfluidics, BioMEMS, and Medical Microsystems XV, 100610O (28 February 2017); https://doi.org/10.1117/12.2250765

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available