Quantifying structural alterations in Alzheimer's disease brains using quantitative phase imaging (Conference Presentation)

Moosung Lee; Eeksung Lee; JaeHwang Jung; Hyeonseung Yu; Kyoohyun Kim; Jonghee Yoon; Shinhwa Lee; Yong Jeong; YongKeun Park

doi:10.1117/12.2251560

24 April 2017 Quantifying structural alterations in Alzheimer's disease brains using quantitative phase imaging (Conference Presentation)

Moosung Lee, Eeksung Lee, JaeHwang Jung, Hyeonseung Yu, Kyoohyun Kim, Jonghee Yoon, Shinhwa Lee, Yong Jeong, YongKeun Park

Author Affiliations +

Proceedings Volume 10074, Quantitative Phase Imaging III; 100741A (2017) https://doi.org/10.1117/12.2251560
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Imaging brain tissues is an essential part of neuroscience because understanding brain structure provides relevant information about brain functions and alterations associated with diseases. Magnetic resonance imaging and positron emission tomography exemplify conventional brain imaging tools, but these techniques suffer from low spatial resolution around 100 μm. As a complementary method, histopathology has been utilized with the development of optical microscopy. The traditional method provides the structural information about biological tissues to cellular scales, but relies on labor-intensive staining procedures. With the advances of illumination sources, label-free imaging techniques based on nonlinear interactions, such as multiphoton excitations and Raman scattering, have been applied to molecule-specific histopathology. Nevertheless, these techniques provide limited qualitative information and require a pulsed laser, which is difficult to use for pathologists with no laser training. Here, we present a label-free optical imaging of mouse brain tissues for addressing structural alteration in Alzheimer’s disease. To achieve the mesoscopic, unlabeled tissue images with high contrast and sub-micrometer lateral resolution, we employed holographic microscopy and an automated scanning platform. From the acquired hologram of the brain tissues, we could retrieve scattering coefficients and anisotropies according to the modified scattering-phase theorem. This label-free imaging technique enabled direct access to structural information throughout the tissues with a sub-micrometer lateral resolution and presented a unique means to investigate the structural changes in the optical properties of biological tissues.

Conference Presentation

Citation Download Citation

Moosung Lee, Eeksung Lee, JaeHwang Jung, Hyeonseung Yu, Kyoohyun Kim, Jonghee Yoon, Shinhwa Lee, Yong Jeong, and YongKeun Park "Quantifying structural alterations in Alzheimer's disease brains using quantitative phase imaging (Conference Presentation)", Proc. SPIE 10074, Quantitative Phase Imaging III, 100741A (24 April 2017); https://doi.org/10.1117/12.2251560

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Brain

Tissues

Neuroimaging

Alzheimer's disease

Phase imaging

Image resolution

Pulsed laser operation

Show All Keywords

Keywords/Phrases

Search In:

Publication Years