Easy-to-use microfluidic chip for long-term 3D-cell cultures

Frank Bunge; Sander van den Driesche; Michael J. Vellekoop

doi:10.1117/12.2265734

30 May 2017 Easy-to-use microfluidic chip for long-term 3D-cell cultures

Frank Bunge, Sander van den Driesche, Michael J. Vellekoop

Proceedings Volume 10247, Bio-MEMS and Medical Microdevices III; 1024706 (2017) https://doi.org/10.1117/12.2265734
Event: SPIE Microtechnologies, 2017, Barcelona, Spain

Abstract

We present a microfluidic chip for an easy setup of a 3D-culture of mammalian cells. The chip contains feeding structures and gas supply for long-term cultivation of mammalian cells. The device is fabricated out of hard materials like silicon and glass that are all highly biocompatible. The chip uses the concept of surficial phaseguides that allows the partial filling of a microfluidic chip with liquids based on hydrophobic and hydrophilic surfaces. Here, a suspension of mammalian cells and melted agarose is filled into the chip and is pulled by the capillary pressure on the hydrophilic areas but not on the hydrophobic phaseguides. Consequently, only a part of the chip is filled with the agarose which gels by cooling a form the 3D-cell culture. The unfilled areas are used as supply structures for nutrition and gases. So the supply is based on diffusion and the supply of nutrition and gases is controlled independently. We cultured HaCaT-cells over 24 hours in our device and achieve a good viability.

Conference Presentation

Citation Download Citation

Frank Bunge, Sander van den Driesche, and Michael J. Vellekoop "Easy-to-use microfluidic chip for long-term 3D-cell cultures", Proc. SPIE 10247, Bio-MEMS and Medical Microdevices III, 1024706 (30 May 2017); https://doi.org/10.1117/12.2265734

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available