Longitudinal spatial coherence gated high-resolution tomography and quantitative phase microscopy of biological cells and tissues with uniform illumination

Dalip Singh Mehta; Azeem Ahmad; Vishesh Dubey; Veena Singh; Ankit Butola; Tonmoy Mohanty; Sreyankar Nandi

doi:10.1117/12.2290334

23 February 2018 Longitudinal spatial coherence gated high-resolution tomography and quantitative phase microscopy of biological cells and tissues with uniform illumination

Dalip Singh Mehta, Azeem Ahmad, Vishesh Dubey, Veena Singh, Ankit Butola, Tonmoy Mohanty, Sreyankar Nandi

Author Affiliations +

Proceedings Volume 10503, Quantitative Phase Imaging IV; 1050305 (2018) https://doi.org/10.1117/12.2290334
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

We report longitudinal spatial coherence (LSC) gated high-resolution tomography and quantitative phase microscopy of biological cells and tissues with uniform illumination using laser as a light source. To accomplish this a pseudo thermal light source was synthesized by passing laser beams through an optical system, which is basically a speckle reduction system with combined effect of spatial, temporal, angular and polarisation diversity. The longitudinal spatial coherence length of such light was significantly reduced by synthesizing a pseudo thermal source with the combined effect of spatial, angular and temporal diversity. This results in a low spatially coherent (i.e., broad angular frequency spectrum) light source with narrow temporal frequency spectrum. Light from such a pseudo thermal light source was passed through an interference microscope with varying magnification, such as, 10X and 50X. The interference microscope was used for full-field OCT imaging of multilayer objects and topography of industrial objects. Experimental results of optical sectioning of multilayer biological objects with high axial-resolution less than 10μm was achieved which is comparable to broadband white light source. The synthesized light source with reduced speckles having uniform illumination on the sample, which can be very useful for fluorescence microscopy as well as quantitative phase microscopy with less phase noise. The present system does not require any dispersion compensation optical system for biological samples as a highly monochromatic light source is used.

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Dalip Singh Mehta, Azeem Ahmad, Vishesh Dubey, Veena Singh, Ankit Butola, Tonmoy Mohanty, and Sreyankar Nandi "Longitudinal spatial coherence gated high-resolution tomography and quantitative phase microscopy of biological cells and tissues with uniform illumination", Proc. SPIE 10503, Quantitative Phase Imaging IV, 1050305 (23 February 2018); https://doi.org/10.1117/12.2290334

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