An immunoturbidimetric assay for specific proteins identification from whole blood based on multi-layered centrifugal microfluidic chip

Jiachen Yang; Kangkang Liu; Minghao Yang; Guanghui Wang

doi:10.1117/12.2549593

20 December 2019 An immunoturbidimetric assay for specific proteins identification from whole blood based on multi-layered centrifugal microfluidic chip

Jiachen Yang, Kangkang Liu, Minghao Yang, Guanghui Wang

Author Affiliations +

Proceedings Volume 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019); 112094F (2019) https://doi.org/10.1117/12.2549593
Event: Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 2019, Xi'an, China

Abstract

In this study, a portable and automatic immunoturbidimetric assay system based on multi-layered centrifugal microfluidics was developed for specific proteins identification from whole blood. With the development of society and medical technology, more and more immune system diseases have been noticed by people. Immune dysfunction in the human body can cause many immune system diseases. Therefore, the detection of specific proteins closely related to immune system diseases has been increasingly favored and paid more attention by medical and scientific researchers. However, for most automated biochemical analyzers, pretreatment and diagnosis process are separated, cannot provide a sample-to-answer solution. To solve this problem, we proposed a highly-integrated system which enables real-time sedimentation of blood cells by centrifugation and quantitative extraction of purified plasma by siphon valve. Also, it has calibration system of standard curves, as well as integrated optical detection device, leading to smaller error fluctuations. As a demonstration experiment, quantification of Immunoglobulin A (IgA) in human whole blood with our LOAD system was conducted. Calibrator sets with specified concentrations ranging from 0.6 to 6 g/L were first generated in real time on the chip. Then standard curves used for IgA quantitation were created. Our LOAD system was demonstrated to have good accuracy and high repeatability since the experimental results of IgA obtained from the LOAD system correlated very well with those from the standard automatic biochemical analyzer method. Furthermore, the LOAD system could conduct simultaneous detection of various specific proteins in the later stage.

Citation Download Citation

Jiachen Yang, Kangkang Liu, Minghao Yang, and Guanghui Wang "An immunoturbidimetric assay for specific proteins identification from whole blood based on multi-layered centrifugal microfluidic chip", Proc. SPIE 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 112094F (20 December 2019); https://doi.org/10.1117/12.2549593

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Blood

Proteins

Microfluidics

Plasma

Calibration

Capillaries

Biological research

Show All Keywords

Keywords/Phrases

Search In:

Publication Years