Label-free multispectral identification of native fluorophores to assess embryo and oocyte quality

Saabah B. Mahbub; Jared Campbell; Tiffany C. Y. Tan; Abbas Habibalahi; Michael J. Bertoldo; Dale M. Goss; Kirsty Walter; Lindsay E. Wu; Kylie R. Dunning; Ewa Goldys

doi:10.1117/12.2579141

5 March 2021 Label-free multispectral identification of native fluorophores to assess embryo and oocyte quality

Saabah B. Mahbub, Jared Campbell, Tiffany C. Y. Tan, Abbas Habibalahi, Michael J. Bertoldo, Dale M. Goss, Kirsty Walter, Lindsay E. Wu, Kylie R. Dunning, Ewa Goldys

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Proceedings Volume 11647, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX; 116471C (2021) https://doi.org/10.1117/12.2579141
Event: SPIE BiOS, 2021, Online Only

Abstract

Our approach to multispectral fluorescence microscopy can non-invasively identify the biomolecular composition of cells and capture complex biological heterogeneity which is fundamentally important for biological research and medical diagnostics. We have applied this technology to demonstrate the embryo quality for chromosomal abnormalities (containing euploid and aneuploidy cells) and understanding the biochemical signatures of polycystic ovarian syndrome (PCOS) oocytes. We then explored oocyte quality following treatment with the NAD+ precursor NMN. These findings demonstrate the utility of our approach to the multispectral assessment of autofluorescence for the non-destructive, label-free assessment of clinically relevant problems.

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Saabah B. Mahbub, Jared Campbell, Tiffany C. Y. Tan, Abbas Habibalahi, Michael J. Bertoldo, Dale M. Goss, Kirsty Walter, Lindsay E. Wu, Kylie R. Dunning, and Ewa Goldys "Label-free multispectral identification of native fluorophores to assess embryo and oocyte quality", Proc. SPIE 11647, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX, 116471C (5 March 2021); https://doi.org/10.1117/12.2579141

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KEYWORDS

Biological research

Analytical research

Hyperspectral imaging

Luminescence

Medical diagnostics

Microscopy

Nondestructive evaluation

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