Local flash photolysis of caged Ca2+ provokes alterations of impulse generation and propagation of cardiac tissue

Kentaro Mochizuki; Yuma Morishita; Shoko Tamura; Hideo Tanaka

doi:10.1117/12.2633241

3 October 2022 Local flash photolysis of caged Ca²⁺ provokes alterations of impulse generation and propagation of cardiac tissue

Kentaro Mochizuki, Yuma Morishita, Shoko Tamura, Hideo Tanaka

Author Affiliations +

Proceedings Volume 12216, Novel Optical Systems, Methods, and Applications XXV; 122160C (2022) https://doi.org/10.1117/12.2633241
Event: SPIE Optical Engineering + Applications, 2022, San Diego, California, United States

Abstract

Cardiomyocyte Ca²⁺ overload is known to disrupt cardiac excitation/conduction; however, unknown is how large area of Ca²⁺-overloaded myocardium is required to provoke such abnormalities. We sought to visualize patterns of excitation/conduction in a cardiomyocyte monolayer before and after localized flash photolysis of caged Ca²⁺. Under electrical stimulation at 2-5 Hz and Fluo8 fluorescence imaging, the monolayer exhibited spatiotemporally uniform propagation of Ca²⁺ transients. Upon UV-light flash (365nm, 70mW/cm^2, 20-250mm^2) the monolayer exhibited increases in Ca²⁺-transient amplitude with occasional emergence of automatic excitation and/or conduction delay on the flashed area. This experimental approach may provide deeper insights into understanding cardiac arrhythmias.

Conference Presentation

Citation Download Citation

Kentaro Mochizuki, Yuma Morishita, Shoko Tamura, and Hideo Tanaka "Local flash photolysis of caged Ca²⁺ provokes alterations of impulse generation and propagation of cardiac tissue", Proc. SPIE 12216, Novel Optical Systems, Methods, and Applications XXV, 122160C (3 October 2022); https://doi.org/10.1117/12.2633241

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