Investigating opioid-modulated receptor heterodimerization by two-photon fluorescence microscopy

Ming-Chi Chen; Cynthia Wei-Sheng Lee; Guan-Yu Zhuo

doi:10.1117/12.3008159

20 September 2023 Investigating opioid-modulated receptor heterodimerization by two-photon fluorescence microscopy

Ming-Chi Chen, Cynthia Wei-Sheng Lee, Guan-Yu Zhuo

Proceedings Volume 12608, Biomedical Imaging and Sensing Conference; 126081H (2023) https://doi.org/10.1117/12.3008159
Event: Optics and Photonics International Congress, 2023, Yokohama, Japan

Abstract

We overexpressed fluorescence-tagged receptors (MOP-CFP & NOP-YFP) by transiently transfecting HEK 293 cells and then measured the variations in fluorescent signals in single-particle tracking (SPT) and fluorescence resonance energy transfer (FRET) by two-photon fluorescence microscopy to explore the cellular localization of μ-opioid (MOP) and nociceptin/orphanin FQ (NOP) receptors regulated by opioids.

Citation Download Citation

Ming-Chi Chen, Cynthia Wei-Sheng Lee, and Guan-Yu Zhuo "Investigating opioid-modulated receptor heterodimerization by two-photon fluorescence microscopy", Proc. SPIE 12608, Biomedical Imaging and Sensing Conference, 126081H (20 September 2023); https://doi.org/10.1117/12.3008159

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