Multi-material integration in light-based volumetric bioprinting: pathways to enhanced precision and complexity in scaffold fabrication

Sammy Florczak; Davide Ribezzi; Marc Falandt; Paulina Bernal; Gabriel Groessbacher; Tina Vermonden; Jos Malda; Riccardo Levato

doi:10.1117/12.3008912

13 March 2024 Multi-material integration in light-based volumetric bioprinting: pathways to enhanced precision and complexity in scaffold fabrication

Sammy Florczak, Davide Ribezzi, Marc Falandt, Paulina Bernal, Gabriel Groessbacher, Tina Vermonden, Jos Malda, Riccardo Levato

Author Affiliations +

Proceedings Volume 12898, Advanced Fabrication Technologies for Micro/Nano Optics and Photonics XVII; 128980G (2024) https://doi.org/10.1117/12.3008912
Event: SPIE OPTO, 2024, San Francisco, California, United States

Abstract

In regenerative medicine, layer-by-layer additive manufacturing has been pivotal in developing intricate 3D tissue scaffolds, yet challenges remain in the fast production of cell-laden structures of clinically relevant (centimeter-scale) sizes. Volumetric Bioprinting (VBP) is a recent optical additive manufacturing technique which facilitates rapid creation of such structures by using spatial light modulation to deliver precise tomographic patterns into a rotating volume of cellladen photoresin, thus allowing for rapid, volumetric crosslinking of materials. Our research enhances VBP by integrating extrusion and electrohydrodynamic printing, thus optimizing multi-cell and multi-material constructs. Using photoresponsive biopolymers and polycaprolactone-based meshes, we have crafted complex cell-laden 3D forms with VBP, introducing diverse features unseen with conventional techniques. With applications for multi-walled blood vessel engineering and specialized cell growth platforms, our findings emphasize the transformative role of optics in biofabrication, suggesting VBP's potential in replicating tissue intricacies and advancing regenerative medicine.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Sammy Florczak, Davide Ribezzi, Marc Falandt, Paulina Bernal, Gabriel Groessbacher, Tina Vermonden, Jos Malda, and Riccardo Levato "Multi-material integration in light-based volumetric bioprinting: pathways to enhanced precision and complexity in scaffold fabrication", Proc. SPIE 12898, Advanced Fabrication Technologies for Micro/Nano Optics and Photonics XVII, 128980G (13 March 2024); https://doi.org/10.1117/12.3008912

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