High-resolution x-ray stereomicroscopy: true three-dimensional imaging of biological samples

Billy W. Loo Jr.; Shawn P. Williams; W. Lin; Willaim H. Love; Stanley Meizel; Stephen S. Rothman

doi:10.1117/12.138755

13 January 1993 High-resolution x-ray stereomicroscopy: true three-dimensional imaging of biological samples

Billy W. Loo Jr., Shawn P. Williams, W. Lin, Willaim H. Love, Stanley Meizel, Stephen S. Rothman

Proceedings Volume 1741, Soft X-Ray Microscopy; (1993) https://doi.org/10.1117/12.138755
Event: San Diego '92, 1992, San Diego, CA, United States

Abstract

X-ray microscopy has the potential to become a powerful tool for the study of biological samples, allowing the imaging of intact cells and subcellular organelles in an aqueous environment at resolutions previously achievable only by electron microscopy. The ability to examine a relatively thick sample raises the issue of superposition of objects from multiple planes within the sample, making difficult the interpretation of conventional, orthogonally projected images. This paper describes our early attempts at developing three-dimensional methods for x-ray microimaging: the first to use x-ray optics, and to our knowledge, the first demonstrating sub-visible resolutions and natural contrast. These studies were performed using the scanning transmission x-ray microscope (STXM) at the National Synchrotron Light Source, Brookhaven National Laboratory.

Citation Download Citation

Billy W. Loo Jr., Shawn P. Williams, W. Lin, Willaim H. Love, Stanley Meizel, and Stephen S. Rothman "High-resolution x-ray stereomicroscopy: true three-dimensional imaging of biological samples", Proc. SPIE 1741, Soft X-Ray Microscopy, (13 January 1993); https://doi.org/10.1117/12.138755

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available