Phase contrast and DIC instrumentation and applications in cell, developmental, and marine biology

Heinz Gundlach

doi:10.1117/12.171868

3 May 1994 Phase contrast and DIC instrumentation and applications in cell, developmental, and marine biology

Heinz Gundlach

Proceedings Volume 1846, Phase Contrast and Differential Interference Contrast Imaging Techniques and Applications; (1994) https://doi.org/10.1117/12.171868
Event: Phase Contrast and Differential Interference Contrast Imaging Techniques and Applications, 1992, Warsaw, Poland

Abstract

Nomarski's differential interference contrast (DIC) microscopy is discussed in comparison to Zernike's phase contrast (PhC) microscopy. The possibilities and limits of both are demonstrated by various applications. The high contrast and the use of the full numerical aperture of the DIC optics makes it possible to obtain a series of 'optical sections' through rather thick living specimens (e.g. head of water flea, salivary gland of Drosophila, Xenopus nucleolus, sea urchen egg, mouse embryo). PhC and DIC optics are today available for high resolution light microscopy until N.A. 1.4 Oil as well as for long working distance (LWD) optics, mainly combined with inverted biological microscopes.

Citation Download Citation

Heinz Gundlach "Phase contrast and DIC instrumentation and applications in cell, developmental, and marine biology", Proc. SPIE 1846, Phase Contrast and Differential Interference Contrast Imaging Techniques and Applications, (3 May 1994); https://doi.org/10.1117/12.171868

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