Fluorescence spectroscopy of bacteriorhodopsin at room temperature

Atanaska Andreeva; Villen Kolev; Tzvetana Lazarova

doi:10.1117/12.320967

19 August 1998 Fluorescence spectroscopy of bacteriorhodopsin at room temperature

Atanaska Andreeva, Villen Kolev, Tzvetana Lazarova

Proceedings Volume 3573, OPTIKA '98: 5th Congress on Modern Optics; (1998) https://doi.org/10.1117/12.320967
Event: OPTIKA '98: Fifth Congress on Modern Optics, 1998, Budapest, Hungary

Abstract

The chromophore fluorescence of bacteriorhodopsin (BR) has been studied by using visible lines of an argon and He-Ne laser at room temperature. This fluorescence is extremely weak and appears as an unstructured band in the 650 - 880 nm range. The same line shape of the emission spectra of dark- and light-adapted BR recorded at low light excitation intensity implies that the emission of 13-cis conformation of retinal is not observable at these conditions. The analysis of the spectra recorded at different pH, intensity and wavelength of excitation light show that the spectrum of BR in fact contains at least three emission bands. They can be assigned to all-trans BR, O and Q intermediates, accumulated during the photocycle. As a most probable emission due to only all-trans BR in light-adapted membrane, we assign a band with a maximum located at 730 nm (13700 cm^-1) and a full width of half maximum equals 2580 cm^-1.

Citation Download Citation

Atanaska Andreeva, Villen Kolev, and Tzvetana Lazarova "Fluorescence spectroscopy of bacteriorhodopsin at room temperature", Proc. SPIE 3573, OPTIKA '98: 5th Congress on Modern Optics, (19 August 1998); https://doi.org/10.1117/12.320967

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