Polymer-based microfluidic devices for biomedical applications

Mateusz L. Hupert; Malgorzata A. Witek; Yun Wang; Michael W. Mitchell; Xuezhu Liu; Yannick Bejat; Dimitris E. Nikitopoulos; Jost Goettert; Michael C. Murphy; Steven A. Soper

doi:10.1117/12.480771

17 January 2003 Polymer-based microfluidic devices for biomedical applications

Mateusz L. Hupert, Malgorzata A. Witek, Yun Wang, Michael W. Mitchell, Xuezhu Liu, Yannick Bejat, Dimitris E. Nikitopoulos, Jost Goettert, Michael C. Murphy, Steven A. Soper

Author Affiliations +

Proceedings Volume 4982, Microfluidics, BioMEMS, and Medical Microsystems; (2003) https://doi.org/10.1117/12.480771
Event: Micromachining and Microfabrication, 2003, San Jose, CA, United States

Abstract

Two types of Microfluidic bioanalytical systems were designed and fabricated in polymer substrates using the LIGA process. A continuous flow polymerase chain reaction (CFPCR) Microfluidic device was fabricated in polycarbonate (PC), which utilized isothermal zone and shuttling the sample through each zone to achieve amplification. A 20-cycle PCR amplification of a fragment of a plasmid DNA template was achieved in 5.3 min. The results were comparable to those obtained in commercial laboratory-scale PCR system. The second system consisted of a microchip contating a low-density array assembled into the Microfluidic channel, which was hot-embossed in poly(methyl methacrylate) (PMMA). The detection of low-abundant mutations in gene fragments (K-ras) that carry point mutations with high diagnostic value for colorectal cancer was successfully performed. The array accessed microfluidics in order to enhance the kinetic associated with hybridization.

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Mateusz L. Hupert, Malgorzata A. Witek, Yun Wang, Michael W. Mitchell, Xuezhu Liu, Yannick Bejat, Dimitris E. Nikitopoulos, Jost Goettert, Michael C. Murphy, and Steven A. Soper "Polymer-based microfluidic devices for biomedical applications", Proc. SPIE 4982, Microfluidics, BioMEMS, and Medical Microsystems, (17 January 2003); https://doi.org/10.1117/12.480771

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