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Nanofabrication is an attractive tool for precise construction of micro/nanostructures to realize fluorescence sensors that may overcome problems related to use of free dyes for biological analysis. Sensor films deposited on optically-functional nanotemplates (<300nm) are proposed as intracellular chemical sensors that provide an internal standard and stable separation of host from toxic foreign materials. Fluorescent nanoparticles were used as templates and intensity references for oxygen- and pH-sensitive fluorophores [Ru(dpp), HPTS], and potassium and sodium-binding dyes (PBFI, Sodium Green). Nanoassembled films on particles were characterized in terms of response to target analytes. These findings suggest that self-assembled nanoparticle sensors may be easily produced and employed as useful tools for real-time cellular analysis.
J. Quincy Brown,Kyle B. Guice,Ryan T. Simpson, andMichael J. McShane
"Electrostatic self-assembly of nanocomposite hybrid fluorescent sensors", Proc. SPIE 5331, Nanobiophotonics and Biomedical Applications, (1 June 2004); https://doi.org/10.1117/12.529793
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J. Quincy Brown, Kyle B. Guice, Ryan T. Simpson, Michael J. McShane, "Electrostatic self-assembly of nanocomposite hybrid fluorescent sensors," Proc. SPIE 5331, Nanobiophotonics and Biomedical Applications, (1 June 2004); https://doi.org/10.1117/12.529793