Fluorescence calibration research of microspheres in a microfluidic chip

Lin Wang; Kun Lian; Jost Goettert

doi:10.1117/12.591610

22 January 2005 Fluorescence calibration research of microspheres in a microfluidic chip

Lin Wang, Kun Lian, Jost Goettert

Proceedings Volume 5718, Microfluidics, BioMEMS, and Medical Microsystems III; (2005) https://doi.org/10.1117/12.591610
Event: MOEMS-MEMS Micro and Nanofabrication, 2005, San Jose, California, United States

Abstract

Fluorescence calibration is usually done by preparing bio-samples with a series of concentrations and measuring their corresponding fluorescence intensities. A simplified approach is studied by using a microfluidic chip and microspheres. The fluorescence calibration can be carried out on the chip with only one concentration of the microspheres. Microspheres with the diameter of 1~5μm are very useful in bio-detection research. These microspheres are manufactured using high-quality, ultraclean polystyrene microspheres and loaded with a variety of proprietary dyes. They can be labeled with biotin-, NeutrAvdin-, streptavidin- and protein, which can be used as tracers for bio-detections. A microfluidic chip was successfully fabricated for the experiment, and preliminary experimental results have proved the feasibility of the approach for fluorescence calibration.

Citation Download Citation

Lin Wang, Kun Lian, and Jost Goettert "Fluorescence calibration research of microspheres in a microfluidic chip", Proc. SPIE 5718, Microfluidics, BioMEMS, and Medical Microsystems III, (22 January 2005); https://doi.org/10.1117/12.591610

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