Investigation of the wavelength dependence of SHG from various tissues

H. J. Askew; S. J. Matcher

doi:10.1117/12.911436

9 February 2012 Investigation of the wavelength dependence of SHG from various tissues

H. J. Askew, S. J. Matcher

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 82261G (2012) https://doi.org/10.1117/12.911436
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

The wavelength-dependent SHG emission efficiency in the epi-direction has been measured for bovine tendon, bovine intervertebral disk, various polymer scaffolds and fixed human decellularized dermis. Whilst qualitative similarities are found for the biological tissues, quantitative differences exist also. The wavelength dependence of SHG from common polymer scaffolds is substantially different to that of bovine tendon. It is found that formaldehyde fixation of bovine tendon not only produces a large decrease in SHG intensity and increase in autofluorescence but also appears to change the wavelength-dependence of the SHG also, shifting the peak efficiency from 1000 nm down towards 840 nm.

Citation Download Citation

H. J. Askew and S. J. Matcher "Investigation of the wavelength dependence of SHG from various tissues", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82261G (9 February 2012); https://doi.org/10.1117/12.911436

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