The use of acoustic energy to manipulate particles and cells (acoustophoresis) is a well-studied and popular technique in microfluidic microscopy applications. This powerful and gentle method has typically required the construction of costly and labour intensive resonating chambers. A new fabrication method for acoustophoretic resonators is presented, using inexpensive materials and without the need for cleanrooms or the special equipment typically found within them. By utilizing a simple glass and polyimide sandwiching technique, single, bifurcating, and trifurcating microchannels were built and tested. Various half and full wavelength transversal resonators were established in microchannel widths of 300, 600, and 750 μm using 1, 2.5, and 5 MHz ultrasound. In significantly simplifying the fabrication and prototyping of these microfluidic resonators we hope to address some of the major drawbacks preventing acoustophoresis technology from being incorporated into the toolkits of laboratories around the world.
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