Mr. Guan-Wei Li Profile

Guan-Wei Li

at National Taiwan Univ

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Publications (2)

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Proceedings Article | 6 March 2014 Paper

On the sensitivity improvement of a miniaturized label-free electrochemical impedance biosensor

Yi-Ching Kuo, Shin-Ting Chou, Pei-I Tsai, Guan-Wei Li, Chih-Ting Lin, Chih-Kung Lee

Proceedings Volume 8976, 897619 (2014) https://doi.org/10.1117/12.2039103

KEYWORDS: Electrodes, Biosensors, Proteins, Gold, Atomic force microscopy, Computer simulations, Acquisition tracking and pointing, Point-of-care devices, Microfluidics, Resolution enhancement technologies

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Development of point-of-care biosensors continues to gain popularity due to the demand of improving the cost performance in today’s health care. As cardiovascular disease induced death remains on the top 3 death causes for most Asian countries, this paper is to present a high-sensitivity point-of-care biosensor for the detection of cardiovascular disease biomarkers. To meet the point-of-care biosensors requirements, which include characteristics such as small size, low cost, and ease of operation, we adopted electrochemical methods as the basis of detection. The 4-aminothiophenol was adopted as the bio-linkers to facilitate the antibody-antigen interaction. A more stable three-electrode configuration was miniaturized and laid out onto a biochip. A microfluidics subsystem based on opto-piezoelectronic technology was also integrated to create the microfluidic biochip system. To improve the detection sensitivity associated with the reduction in biochip size, electrochemistry simulation was used to investigate several potentially effective means. We found that the electric field on the edge near working electrode and counter electrode was higher, which was verified by using atomic force microscopy to measure the surface potential. With the successful verification, we explored the configuration, i.e., lengthened the edge of working electrode and counter electrode without changing the areas of working electrode and counter electrode and the gap between these two electrodes, so as to evaluate the possibility of improving the measurement efficiency in our newly developed biochips. Detailed design, simulation and experimental results, improved design identified, etc. were all presented in detail.

Proceedings Article | 4 March 2014 Paper

Electrochemical impedance spectroscopy based-on interferon-gamma detection

Guan-Wei Li, Yi-Ching Kuo, Pei-I Tsai, Chih-Kung Lee

Proceedings Volume 8936, 893606 (2014) https://doi.org/10.1117/12.2039188

KEYWORDS: Electrodes, Biosensors, Polarization, Gold, Interferometry, Dielectric spectroscopy, Mirrors, Diagnostics, Transducers, Resistance

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Tuberculosis (TB) is an ancient disease constituted a long-term menace to public health. According to World Health Organization (WHO), mycobacterium tuberculosis (MTB) infected nearly a third of people of the world. There is about one new TB occurrence every second. Interferon-gamma (IFN-γ) is associated with susceptibility to TB, and interferongamma release assays (IGRA) is considered to be the best alternative of tuberculin skin test (TST) for diagnosis of latent tuberculosis infection (LTBI). Although significant progress has been made with regard to the design of enzyme immunoassays for IFN-γ, adopting this assay is still labor-intensive and time-consuming. To alleviate these drawbacks, we used IFN-γ antibody to facilitate the detection of IFN-γ. An experimental verification on the performance of IGRA was done in this research. We developed two biosensor configurations, both of which possess high sensitivity, specificity, and rapid IFN-γ diagnoses. The first is the electrochemical method. The second is a circular polarization interferometry configuration, which incorporates two light beams with p-polarization and s-polarization states individually along a common path, a four photo-detector quadrature configuration to arrive at a phase modulated ellipsometer. With these two methods, interaction between IFN-γ antibody and IFN-γ were explored and presented in detail.

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