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Theoretically, the numerical aperture through the pupil of the mouse eye permits sub-micrometer imaging of the retina. However, optical aberrations introduced by the tear film, cornea and intraocular lens reduce the actual resolution. In order to approach diffraction limited imaging, these aberrations can be corrected with Adaptive Optics (AO) using a wavefront corrector such as Deformable Mirror (DM). The traditional approach to AO is to use a Wavefront Sensor (WFS) to measure the ocular aberrations directly. Performing accurate wavefront measurements for WFS AO imaging in a small animal eye requires a high system complexity due to the short length of the eye creating an optically thick sample with multiple scattering surfaces. Alternatively, Sensorless AO (SAO) has the potential to overcome the limitations of the SH-WFS. SAO does not require direct measurement of the optical wavefront but instead uses an image-based approach. SAO methods have the ability to provide depth resolved aberration correction by performing the aberration correction at different layers within the retina.
Our imaging system provides volumetric TPEF imaging in the retina using SAO-OCT for depth-specific aberration correction, using same light source to generate the OCT and TPEF. Here, we present our progress since our previous report4 with improvements to the light delivery, aberration correction, and TPEF detection.
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