Intravital multiphoton autofluorescence imaging is sensitive to changes in T cell and melanoma tumor cell metabolism during immunotherapy

Alexa R. Heaton; Anna Hoefges; Peter R. Rehani; Angelica F. Lopez; Nathaniel J. Burkard; Alexander L. Rakhmilevich; Amy K Erbe; Paul M. Sondel; Melissa C. Skala

doi:10.1117/12.2607108

2 March 2022 Intravital multiphoton autofluorescence imaging is sensitive to changes in T cell and melanoma tumor cell metabolism during immunotherapy

Alexa R. Heaton, Anna Hoefges, Peter R. Rehani, Angelica F. Lopez, Nathaniel J. Burkard, Alexander L. Rakhmilevich, Amy K Erbe, Paul M. Sondel, Melissa C. Skala

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Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC119720G (2022) https://doi.org/10.1117/12.2607108
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Intravital multiphoton microscopy of the metabolic co-enzymes NAD(P)H and FAD (optical metabolic imaging, or OMI) provides label-free imaging of metabolic changes in vivo. Since the metabolism of tumor and immune cells is associated with cancer progression, we aim to study metabolic changes during a triple-combination immunotherapy regimen that cures murine melanoma tumors. Our results demonstrate that intravital OMI can capture tumor and T cell autofluorescence intensity and lifetime changes during immunotherapy. Overall, this technology enables analysis of single cell metabolic changes in vivo to provide insight for immunotherapy development.

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Alexa R. Heaton, Anna Hoefges, Peter R. Rehani, Angelica F. Lopez, Nathaniel J. Burkard, Alexander L. Rakhmilevich, Amy K Erbe, Paul M. Sondel, and Melissa C. Skala "Intravital multiphoton autofluorescence imaging is sensitive to changes in T cell and melanoma tumor cell metabolism during immunotherapy", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720G (2 March 2022); https://doi.org/10.1117/12.2607108

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KEYWORDS

Tumors

Melanoma

Mode conditioning cables

Auto-fluorescence imaging

In vivo imaging

Single photon

Skin

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