Multi-photon microscopy was used to determine the differential response to radiation at multiple time points between radiation-resistant and -susceptible human head and neck cancer tumor xenografts grown in a cohort of 60 athymic mice. Mice were randomly divided into groups for cell line, treatment or control, and time points relative to treatment — baseline, 1 hour-, 24 hours-, and 48 hours-post-treatment. We quantified the optical redox ratio, lifetime of NAD(P)H, and heterogeneity in ORR and lifetime endpoints. We saw a significant effect of cell line, treatment, and the interaction of all three factors — line, treatment, and time point.
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