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Laser scanning multiphoton microscopy is widely used in biomedical research. The commonly available system can support a frame rate of up to tens of Hz. Recent advances in function indicators demand a frame rate of hundreds of Hz, beyond the capabilities of common systems. Although a few novel solutions have been developed recently, they often require highly specialized sources and hardware. To broadly enable high-speed imaging, we developed an optical gearbox system that is compatible with commonly available hardware. Here, we present the results of the gearbox based high-speed in vivo functional imaging.
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