Real-time assessment of liver optical properties can play a critical role in evaluating liver perfusion and oxygen delivery immediately post a liver transplant and the extent of tissue damage during thermal tumor ablation. Diffuse reflectance spectroscopy in the visible wavelength range (vis-DRS) is a powerful tool for measuring tissue absorption and scattering properties but is challenging to be used on highly absorptive liver tissue. We demonstrated that, with proper design, vis-DRS can be used to reliably quantify liver tissue optical properties, hemoglobin contents, and oxygen saturation, in both ex vivo and in vivo settings.
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