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13 March 2024 3D microfluidic biochips made of amorphas fluoropolymer CYTOP using two-photon polymerized molding towards super-resolution bioimaging
Kotaro Obata, Mirai Hanzawa, Felix Sima, Hiroyuki Kawano, Kozunari Ozasa, Yasutaka Hanada, Godai Miyaji, Atsushi Miyawaki, Koji Sugioka
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Proceedings Volume PC12837, Microfluidics, BioMEMS, and Medical Microsystems XXII; PC1283702 (2024) https://doi.org/10.1117/12.3002296
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Development of biochips enabling distortion-free imaging in the microchannel filled with a culture medium (water) is required to investigate behavior of living cells in micro and nano environments. Fluoropolymer CYTOP is a promising material as a platform of biochips for the distortion-free imaging, because the refractive index of CYTOP (1.34) is almost same as 1.33 of water. In this study, we have developed a new 3D fabrication method for CYTOP by two-photon polymerized structures using a femtosecond laser as molds, which will be used for fabrication of micro and nano environment platforms for super-resolution bioimaging.
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Kotaro Obata, Mirai Hanzawa, Felix Sima, Hiroyuki Kawano, Kozunari Ozasa, Yasutaka Hanada, Godai Miyaji, Atsushi Miyawaki, and Koji Sugioka "3D microfluidic biochips made of amorphas fluoropolymer CYTOP using two-photon polymerized molding towards super-resolution bioimaging", Proc. SPIE PC12837, Microfluidics, BioMEMS, and Medical Microsystems XXII, PC1283702 (13 March 2024); https://doi.org/10.1117/12.3002296
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KEYWORDS
Microfluidics
Polymers
Super resolution
3D microstructuring
Fabrication
Two photon polymerization
Femtosecond phenomena
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