Large chromatic shift objective for chromatic confocal microscopy

Jiabin Chen; Shuyuan Guan; Shaobai Li; Wenjun Kang; Rongguang Liang

doi:10.1117/12.3007669

13 March 2024 Large chromatic shift objective for chromatic confocal microscopy

Jiabin Chen, Shuyuan Guan, Shaobai Li, Wenjun Kang, Rongguang Liang

Proceedings Volume PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; PC1284804 (2024) https://doi.org/10.1117/12.3007669
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The chromatic confocal microscope is an innovative method to acquire axial information simultaneously but suffers from limited imaging depth. We designed an 800um chromatic shift chromatic objective and manufactured it by precision diamond turning. By utilizing this objective, our chromatic confocal system can capture a large depth axis information simultaneously without stage scanning, while maintaining 780um lateral resolution. Biology tissues are visualized under this microscope to evaluate its performance.

Conference Presentation

Citation Download Citation

Jiabin Chen, Shuyuan Guan, Shaobai Li, Wenjun Kang, and Rongguang Liang "Large chromatic shift objective for chromatic confocal microscopy", Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC1284804 (13 March 2024); https://doi.org/10.1117/12.3007669

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