AuNPs amplification in surface plasmon resonance imaging for the detection of micro-RNAs in the context of organ donation

Coline Beltrami; Julien Moreau; Laurence Convert; Jean-François Bryche; Paul G. Charette; Michael Canva

doi:10.1117/12.2692873

13 March 2024 AuNPs amplification in surface plasmon resonance imaging for the detection of micro-RNAs in the context of organ donation

Coline Beltrami, Julien Moreau, Laurence Convert, Jean-François Bryche, Paul G. Charette, Michael Canva

Author Affiliations +

Proceedings Volume PC12860, Plasmonics in Biology and Medicine XXI; PC128600C (2024) https://doi.org/10.1117/12.2692873
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We present a Surface Plasmon Resonance Imaging (SPRI) biochip system to quantitatively detect micro-RNAs involved in the cytokine storm during an inflammatory response. The thiol composition of the self-assembled monolayer on the biochip gold surface was tuned to maximize the capture of RNAs at low concentrations. To further amplify this signal, we have developed a sandwich-like assay using oligonucleotides functionalized gold nanoparticles (AuNPs), synthesized at ambient temperature and optimized to have a high solubility in saline solutions. Sub-picomolar detection limit of those small RNAs was achieved with all these combined improvements.

Conference Presentation

Citation Download Citation

Coline Beltrami, Julien Moreau, Laurence Convert, Jean-François Bryche, Paul G. Charette, and Michael Canva "AuNPs amplification in surface plasmon resonance imaging for the detection of micro-RNAs in the context of organ donation", Proc. SPIE PC12860, Plasmonics in Biology and Medicine XXI, PC128600C (13 March 2024); https://doi.org/10.1117/12.2692873

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