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We present a Surface Plasmon Resonance Imaging (SPRI) biochip system to quantitatively detect micro-RNAs involved in the cytokine storm during an inflammatory response. The thiol composition of the self-assembled monolayer on the biochip gold surface was tuned to maximize the capture of RNAs at low concentrations. To further amplify this signal, we have developed a sandwich-like assay using oligonucleotides functionalized gold nanoparticles (AuNPs), synthesized at ambient temperature and optimized to have a high solubility in saline solutions. Sub-picomolar detection limit of those small RNAs was achieved with all these combined improvements.
Coline Beltrami,Julien Moreau,Laurence Convert,Jean-François Bryche,Paul G. Charette, andMichael Canva
"AuNPs amplification in surface plasmon resonance imaging for the detection of micro-RNAs in the context of organ donation", Proc. SPIE PC12860, Plasmonics in Biology and Medicine XXI, PC128600C (13 March 2024); https://doi.org/10.1117/12.2692873
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Coline Beltrami, Julien Moreau, Laurence Convert, Jean-François Bryche, Paul G. Charette, Michael Canva, "AuNPs amplification in surface plasmon resonance imaging for the detection of micro-RNAs in the context of organ donation," Proc. SPIE PC12860, Plasmonics in Biology and Medicine XXI, PC128600C (13 March 2024); https://doi.org/10.1117/12.2692873