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13 March 2024 Fabrication of 3D CYTOP biochip using two-photon polymerized mold towards super-resolution bioimaging
Mirai Hanzawa, Kotaro Obata, Felix Sima, Hiroyuki Kawano, Kazunari Ozasa, Yasutaka Hanada, Godai Miyaji, Atsushi Miyawaki, Koji Sugioka
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Proceedings Volume PC12872, Laser Applications in Microelectronic and Optoelectronic Manufacturing (LAMOM) XXIX; PC128720D (2024) https://doi.org/10.1117/12.3001200
Event: SPIE LASE, 2024, San Francisco, California, United States
Abstract
As a platform for high resolution bioimaging, biochips with a refractive index very close to that of a culture medium (water) are highly demanded. CYTOP (AGC Corporation) is a promising material, since its refractive index (1.34) is almost similar to that of water (1.33). However, high transparency in also the ultraviolet region and high chemical resistance of CYTOP make it difficult to fabricate 3D microstructures for biochips. In this study we present fabrication method of 3D biochips based on CYTOP for cell observation by the molding process using 3D micro/nanostructures fabricated by two-photon polymerization with femtosecond laser.
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Mirai Hanzawa, Kotaro Obata, Felix Sima, Hiroyuki Kawano, Kazunari Ozasa, Yasutaka Hanada, Godai Miyaji, Atsushi Miyawaki, and Koji Sugioka "Fabrication of 3D CYTOP biochip using two-photon polymerized mold towards super-resolution bioimaging", Proc. SPIE PC12872, Laser Applications in Microelectronic and Optoelectronic Manufacturing (LAMOM) XXIX, PC128720D (13 March 2024); https://doi.org/10.1117/12.3001200
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KEYWORDS
Fabrication
Polymers
Super resolution
Refractive index
Two photon polymerization
Water
Prostate cancer
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