PScan 1.0: flexible software framework for polygon based multiphoton microscopy

Yongxiao Li; Woei Ming Lee

doi:10.1117/12.2242954

9 December 2016 PScan 1.0: flexible software framework for polygon based multiphoton microscopy

Yongxiao Li, Woei Ming Lee

Proceedings Volume 10013, SPIE BioPhotonics Australasia; 1001333 (2016) https://doi.org/10.1117/12.2242954
Event: SPIE BioPhotonics Australasia, 2016, Adelaide, Australia

Abstract

Multiphoton laser scanning microscopes exhibit highly localized nonlinear optical excitation and are powerful instruments for in-vivo deep tissue imaging. Customized multiphoton microscopy has a significantly superior performance for in-vivo imaging because of precise control over the scanning and detection system. To date, there have been several flexible software platforms catered to custom built microscopy systems i.e. ScanImage, HelioScan, MicroManager, that perform at imaging speeds of 30-100fps. In this paper, we describe a flexible software framework for high speed imaging systems capable of operating from 5 fps to 1600 fps. The software is based on the MATLAB image processing toolbox. It has the capability to communicate directly with a high performing imaging card (Matrox Solios eA/XA), thus retaining high speed acquisition. The program is also designed to communicate with LabVIEW and Fiji for instrument control and image processing. Pscan 1.0 can handle high imaging rates and contains sufficient flexibility for users to adapt to their high speed imaging systems.

Citation Download Citation

Yongxiao Li and Woei Ming Lee "PScan 1.0: flexible software framework for polygon based multiphoton microscopy", Proc. SPIE 10013, SPIE BioPhotonics Australasia, 1001333 (9 December 2016); https://doi.org/10.1117/12.2242954

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