Imaging cold-induced vasodynamic behaviour in skin using OCT for microangiography (Conference Presentation)

Malte Casper; Hinnerk Schulz-Hildebrandt; Michael Evers; Cuc Nguyen; Reginald Birngruber; Gereon Hüttmann; Dieter Manstein

doi:10.1117/12.2251485

19 April 2017 Imaging cold-induced vasodynamic behaviour in skin using OCT for microangiography (Conference Presentation)

Malte Casper, Hinnerk Schulz-Hildebrandt, Michael Evers, Cuc Nguyen, Reginald Birngruber, Gereon Hüttmann, Dieter Manstein

Author Affiliations +

Proceedings Volume 10037, Photonics in Dermatology and Plastic Surgery; 100370S (2017) https://doi.org/10.1117/12.2251485
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

In dermatology the reflexes of vasoconstriction and vasodilation are known as important mechanisms of thermoregulation of the inner body. Imaging the physiology of microvasculature of the skin with high spatial resolution in three dimensions while reacting to changes in temperature is crucial for understanding the complex processes of vasodynamics, which result in constriction and dilation of vessels. However, previous studies using Laser-Doppler flowmetry and -imaging could not provide reliable angiographic images which allow to quantify changes in blood vessel diameter. Here, we report a different approach for angiographic imaging of microvasculature of a anaesthetized rodent model using speckle variance optical coherence tomography (svOCT) during and after localized cooling. Therefore a commercial OCT with a center wavelength of 1.3 μm and a spatial resolution of 13µm was used in combination with a custom built cooling device to image such reflexes at the mouse ear pinna and dorsal skinfold. Cooling was applied in steps of 2−5◦ C starting at the baseline temperature of 27◦ C down to −10◦ C. To our surprise and in contrast to the general opinion in literature, we were able to observe that the majority of vessels with a diameter larger than 20 μm maintain perfused with a constant diameter when the tissue is cooled from baseline to subzero temperatures. However, vasoconstriction was observed very rarely and only in veins, which led to their occlusion. The results of this experiment lead us to reconsider essential aspects of previous understanding of temperature-induced vasodynamics in cutaneous microvasculature.

Conference Presentation

Citation Download Citation

Malte Casper, Hinnerk Schulz-Hildebrandt, Michael Evers, Cuc Nguyen, Reginald Birngruber, Gereon Hüttmann, and Dieter Manstein "Imaging cold-induced vasodynamic behaviour in skin using OCT for microangiography (Conference Presentation)", Proc. SPIE 10037, Photonics in Dermatology and Plastic Surgery, 100370S (19 April 2017); https://doi.org/10.1117/12.2251485

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Optical coherence tomography

Skin

Angiography

Spatial resolution

Dermatology

3D image processing

Blood vessels

Show All Keywords

Keywords/Phrases

Search In:

Publication Years