Optical manipulation of hot nanoparticles can mediate selected cell fusion

Lene B. Oddershede; Azra Bahadori; Poul M. Bendix

doi:10.1117/12.2270424

18 April 2017 Optical manipulation of hot nanoparticles can mediate selected cell fusion

Lene B. Oddershede, Azra Bahadori, Poul M. Bendix

Proceedings Volume 10252, Optical Manipulation Conference; 1025207 (2017) https://doi.org/10.1117/12.2270424
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

Metallic nanoparticles with diameters from 10 nm to 250 nm can be optically trapped and manipulated in 3D using a single tightly focused near infrared laser beam. This will result in a significant heating of the particle and its vicinity, with temperature increases easily reaching hundreds degrees Celsius. If such a hot metallic nanoparticle is brought into the contact zone between two cells or vesicles, this local temperature increase can cause a total fusion of the selected cells or vesicles. Upon fusion, both the membrane and the cargos become completely mixed and we also show that the cells remain viable after fusion. The presented method has potential for single-cell targeted drug delivery and for the creation of hybrid cells.

Citation Download Citation

Lene B. Oddershede, Azra Bahadori, and Poul M. Bendix "Optical manipulation of hot nanoparticles can mediate selected cell fusion", Proc. SPIE 10252, Optical Manipulation Conference, 1025207 (18 April 2017); https://doi.org/10.1117/12.2270424

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