Paper
25 August 2017 Microbubble trapping in inverted optical tweezers
Thomas J. Smart, Mehmet Burcin Unlu, Philip H. Jones
Author Affiliations +
Abstract
We have developed an inverted microscope optical tweezers for trapping and manipulation of microscopic gas bubbles. Trapping is achieved by a time-averaged optical trap using a rapidly-scanning Gaussian laser beam. Unlike holographic optical tweezers for microbubbles that employ a Laguerre-Gaussian beam, in this configuration the backwards-directed optical gradient force is sufficient to confine a microbubble against both the optical scattering force and the microbubble buoyancy. We have calibrated the optical trapping forces for microbubbles with a range of sizes, and determined the scanning trap configuration that produces the strongest confinement. Our system also includes a real-time “point-and-click” user interface for interactive selection, capture and isolation of individual microbubbles with optimal trap stiffness.
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Thomas J. Smart, Mehmet Burcin Unlu, and Philip H. Jones "Microbubble trapping in inverted optical tweezers", Proc. SPIE 10347, Optical Trapping and Optical Micromanipulation XIV, 1034731 (25 August 2017); https://doi.org/10.1117/12.2274033
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KEYWORDS
Optical tweezers

Calibration

Geometrical optics

Holography

Human-machine interfaces

Laser scattering

Microscopes

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