Micro-array isolation of circulating tumor cells (CTCs): the droplet biopsy chip

B. Panchapakesan

doi:10.1117/12.2275048

29 August 2017 Micro-array isolation of circulating tumor cells (CTCs): the droplet biopsy chip

B. Panchapakesan

Proceedings Volume 10352, Biosensing and Nanomedicine X; 103520G (2017) https://doi.org/10.1117/12.2275048
Event: SPIE Nanoscience + Engineering, 2017, San Diego, California, United States

Abstract

We present a new method for circulating tumor cell capture based on micro-array isolation from droplets. Called droplet biopsy, our technique uses a 76-element array of carbon nanotube devices functionalized with anti-EpCAM and antiHer2 antibodies for immunocapture of spiked breast cancer cells in the blood. This droplet biopsy chip can enable capture of CTCs based on both positive and negative selection strategy. Negative selection is achieved through depletion of contaminating leukocytes through the differential settling of blood into layers. We report 55%-100% cancer cell capture yield in this first droplet biopsy chip study. The droplet biopsy is an enabling idea where one can capture CTCs based on multiple biomarkers in a single blood sample.

Conference Presentation

Citation Download Citation

B. Panchapakesan "Micro-array isolation of circulating tumor cells (CTCs): the droplet biopsy chip", Proc. SPIE 10352, Biosensing and Nanomedicine X, 103520G (29 August 2017); https://doi.org/10.1117/12.2275048

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