Fluorescence assessment of the delivery and distribution of nebulized indocyanine green in a murine model

Giulia Kassab; Mariana C. Geralde; Natalia M. Inada; Vanderlei S. Bagnato

doi:10.1117/12.2293013

7 February 2018 Fluorescence assessment of the delivery and distribution of nebulized indocyanine green in a murine model

Giulia Kassab, Mariana C. Geralde, Natalia M. Inada, Vanderlei S. Bagnato

Author Affiliations +

Proceedings Volume 10475, Visualizing and Quantifying Drug Distribution in Tissue II; 104750M (2018) https://doi.org/10.1117/12.2293013
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Photodynamic inactivation (PDI) is a promising alternative for the treatment of infectious diseases, and the combination of indocyanine green (ICG) and extracorporeal infrared light has shown optimistic results against pneumonia in vitro and in vivo. However, the pharmacokinetics and the possible side effects of the pulmonary delivery via nebulization have not been fully investigated. This study assessed the distribution of the photosensitizer within the lungs and to other organs of mice, and monitored the fluorescence of ICG in the thorax in the presence and absence of the activating light. The excitation wavelength was 780 nm and detection focused on the emission between 795 and 890 nm. Experiments demonstrated that the amount of fluorescence detected from outside the body was significantly higher after the nebulization of ICG, and reduced after the illumination, allowing for the monitoring of the PDI in real time. The fluorescence remained detectable in the mice for at least 24 hours, and was present in the lungs, stomach, liver, small and large intestines, bladder, spleen and heart after this time.

Citation Download Citation

Giulia Kassab, Mariana C. Geralde, Natalia M. Inada, and Vanderlei S. Bagnato "Fluorescence assessment of the delivery and distribution of nebulized indocyanine green in a murine model", Proc. SPIE 10475, Visualizing and Quantifying Drug Distribution in Tissue II, 104750M (7 February 2018); https://doi.org/10.1117/12.2293013

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE