In-vivo three-dimensional optoacoustic molecular imaging of the synovial vasculature in a mouse model of knee joint osteoarthritis (Conference Presentation)

Zhongyu LIU; Xuan Wang; Manting Au; Duojia Li; Yongping Zheng; Chunxiao Luo; Limin Rong; Chunyi Wen

doi:10.1117/12.2299658

15 March 2018 In-vivo three-dimensional optoacoustic molecular imaging of the synovial vasculature in a mouse model of knee joint osteoarthritis (Conference Presentation)

Zhongyu LIU, Xuan Wang, Manting Au, Duojia Li, Yongping Zheng, Chunxiao Luo, Limin Rong, Chunyi Wen

Author Affiliations +

Proceedings Volume 10494, Photons Plus Ultrasound: Imaging and Sensing 2018; 104940L (2018) https://doi.org/10.1117/12.2299658
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Synovitis is a driver of osteoarthritis. Imaging of the synovial vasculature is essential for osteoarthritis assessment, while traditional imaging techniques like contrast-enhanced MRI/CT and conventional ultrasonography have limitations such as invasive manipulation, radiation and subjective results. In this study, an emerging non-invasive ultrasound imaging technique – optoacoustic molecular imaging (OA) was applied to evaluate the synovial vasculature in a mouse model of knee joint osteoarthritis. 16 male Balb/c mice undertook destabilization of medial meniscus surgery (DMM), 8 were intact as baseline controls. Three-dimensional high-frequency ultrasonography, including B mode, Power Doppler (PD) and OA, was performed to the knees of live mice at baseline (n=8), 1 month (n=8), and 4 months (n=7) after DMM, before tissue harvest. We found that OA vascular density increased significantly at 1 month (p=0.028) and remained high at 4 months (p=0.541), indicating synovial neovascularization during osteoarthritis progression, which was consistent with uCT-based angiography and histological findings. Meanwhile, OA could also evaluate the function of the synovial vasculature by measuring blood oxygen saturation (sO2). We found OA sO2 declined significantly at 4 months compared to baseline (p=0.043) and 1 month (p=0.027), indicating vascular dysfunction at late stage osteoarthritis. Moreover, OA sO2 was found closely related with histological cartilage damage (p=0.028). In this study, we demonstrated that OA was reliable to evaluate the small vasculatures in the knee joint of DMM mouse model. Our findings provided a new technique for the non-invasive monitoring of both structure and function of the synovial vasculature during osteoarthritis progression.

Conference Presentation

Citation Download Citation

Zhongyu LIU, Xuan Wang, Manting Au, Duojia Li, Yongping Zheng, Chunxiao Luo, Limin Rong, and Chunyi Wen "In-vivo three-dimensional optoacoustic molecular imaging of the synovial vasculature in a mouse model of knee joint osteoarthritis (Conference Presentation)", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104940L (15 March 2018); https://doi.org/10.1117/12.2299658

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Mouse models

Molecular imaging

3D image processing

3D modeling

In vivo imaging

Ultrasonography

Control systems

Show All Keywords

Keywords/Phrases

Search In:

Publication Years