Rapid in vivo vertical tissue sectioning by multiphoton tomography

Ana Batista; Hans Georg Breunig; Karsten König

doi:10.1117/12.2286915

23 February 2018 Rapid in vivo vertical tissue sectioning by multiphoton tomography

Ana Batista, Hans Georg Breunig, Karsten König

Author Affiliations +

Proceedings Volume 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII; 1049814 (2018) https://doi.org/10.1117/12.2286915
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

A conventional tool in the pathological field is histology which involves the analysis of thin sections of tissue in which specific cellular structures are stained with different dyes. The process to obtain these stained tissue sections is time consuming and invasive as it requires tissue removal, fixation, sectioning, and staining. Moreover, imaging of live tissue is not possible. We demonstrate that multiphoton tomography can provide within seconds, non-invasive, label-free, vertical images of live tissue which are in quality similar to conventional light micrographs of histologic stained specimen. In contrast to conventional setups based on laser scanning which image horizontally sections, the vertical in vivo images are directly recorded by combined line scanning and timed adjustments of the height of the focusing optics. In addition, multiphoton tomography provides autofluorescence lifetimes which can be used to determine the metabolic states of cells.

Citation Download Citation

Ana Batista, Hans Georg Breunig, and Karsten König "Rapid in vivo vertical tissue sectioning by multiphoton tomography", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 1049814 (23 February 2018); https://doi.org/10.1117/12.2286915

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