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20 February 2018 Interferometric scattering (iSCAT) microscopy: studies of biological membrane dynamics
Francesco Reina, Silvia Galiani, Dilip Shrestha, Erdinc Sezgin, B. Christoffer Lagerholm, Daniel Cole, Philipp Kukura, Christian Eggeling
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Proceedings Volume 10500, Single Molecule Spectroscopy and Superresolution Imaging XI; 105000L (2018) https://doi.org/10.1117/12.2289501
Event: SPIE BiOS, 2018, San Francisco, California, United States
Abstract
The study of the organization and dynamics of molecules in model and cellular membranes is an important topic in contemporary biophysics. Imaging and single particle tracking in this particular field, however, proves particularly demanding, as it requires simultaneously high spatio-temporal resolution and high signal-to-noise ratios. A remedy to this challenge might be Interferometric Scattering (iSCAT) microscopy, due to its fast sampling rates, label-free imaging capabilities and, most importantly, tuneable signal level output. Here we report our recent advances in the imaging and molecular tracking on phase-separated model membrane systems and live-cell membranes using this technique.
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Francesco Reina, Silvia Galiani, Dilip Shrestha, Erdinc Sezgin, B. Christoffer Lagerholm, Daniel Cole, Philipp Kukura, and Christian Eggeling "Interferometric scattering (iSCAT) microscopy: studies of biological membrane dynamics", Proc. SPIE 10500, Single Molecule Spectroscopy and Superresolution Imaging XI, 105000L (20 February 2018); https://doi.org/10.1117/12.2289501
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KEYWORDS
Diffusion
Gold
Scattering
Microscopy
Nanoparticles
Interferometry
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