Label-free in vitro prostate cancer cell detection via photonic-crystal biosensor

Frank DeLuna; XiaoFei Ding; Ismael Sagredo; Gilbert Bustamante; Lu-Zhe Sun; Jing Yong Ye

doi:10.1117/12.2288019

20 February 2018 Label-free in vitro prostate cancer cell detection via photonic-crystal biosensor

Frank DeLuna, XiaoFei Ding, Ismael Sagredo, Gilbert Bustamante, Lu-Zhe Sun, Jing Yong Ye

Proceedings Volume 10504, Biophysics, Biology and Biophotonics III: the Crossroads; 105040D (2018) https://doi.org/10.1117/12.2288019
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Prostate-specific antigen (PSA) biomarker assays are the current clinical method for mass screening of prostate cancer. However, high false-positive rates are often reported due to PSA’s low specificity, leading to an urgent need for the development of a more specific detection system independent of PSA levels. In our previous research, we demonstrated the feasibility of using cellular refractive indices (RI) as a unique contrast parameter to accomplish label-free detection of prostate cancer cells via variance testing, but were unable to determine if a specific cell was cancerous or noncancerous. In this paper, we report the use of our Photonic-Crystal biosensor in a Total-Internal-Reflection (PC-TIR) configuration to construct a label-free imaging system, which allows for the detection of individual prostate cancer cells utilizing cellular RI as the only contrast parameter. Noncancerous prostate (BPH-1) cells and prostate cancer (PC-3) cells were mixed at varied ratios and measured concurrently. Additionally, we isolated and induced PC-3 cells to undergo epithelial-mesenchymal transition (EMT) by exposing these cells to soluble factors such as TGF-β1. The biophysical characteristics of the cellular RI were quantified extensively in comparison to non-induced PC-3 cells as well as BPH-1 cells. EMT is a crucial mechanism for the invasion and metastasis of epithelial tumors characterized by the loss of cell-cell adhesion and increased cell mobility. Our study shows promising clinical potential in utilizing the PC-TIR biosensor imaging system to not only detect prostate cancer cells, but also evaluate prostate cancer progression.

Conference Presentation

Citation Download Citation

Frank DeLuna, XiaoFei Ding, Ismael Sagredo, Gilbert Bustamante, Lu-Zhe Sun, and Jing Yong Ye "Label-free in vitro prostate cancer cell detection via photonic-crystal biosensor", Proc. SPIE 10504, Biophysics, Biology and Biophotonics III: the Crossroads, 105040D (20 February 2018); https://doi.org/10.1117/12.2288019

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