Effect of linkers on the αvβ3 integrin targeting efficiency of cyclic RGD-conjugates.

Partha Karmakar; Dorota Grabowska; Gail Sudlow; Kostiantyn Ziabrev; Nibedita Sanyal; Samuel Achilefu

doi:10.1117/12.2301223

20 February 2018 Effect of linkers on the α_vβ₃ integrin targeting efficiency of cyclic RGD-conjugates

Partha Karmakar, Dorota Grabowska, Gail Sudlow, Kostiantyn Ziabrev, Nibedita Sanyal, Samuel Achilefu

Proceedings Volume 10508, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X; 1050809 (2018) https://doi.org/10.1117/12.2301223
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Cyclic arginine-glycine-aspartic acid (cRGD) peptides are well known to target α_νβ₃ integrin expressed on cancer cells and neovasculature. Conjugation of these peptides with dyes, drugs, antibodies and other biomolecules through covalent linkers provides a facile way to deliver these products to tumor cells for targeted cancer therapy and diagnosis. Click chemistry and acid-amine couplings are widely used conjugation strategies. However, the effects of different linkers and the distance between the cRGD and the conjugates on the binding of cRGD ligand with α_νβ₃ has been underexplored.

In this present study, we prepared cRGD-conjugates using different linkers and determined how they altered the tumor targeting efficiency in vitro and in vivo. The results demonstrate that different linkers significantly altered the pharmacokinetics of the cRGD conjugates and the tumor uptake kinetics. Unlike large antibodies, this preliminary finding shows that linkers used to attach drugs and fluorescent molecular probes to small peptides play a major role in the accuracy of tumor targeting and treatment outcomes. As a result, considerable attention should be paid to the nature of linkers used in the design of molecular probes and targeted therapeutics.

Citation Download Citation

Partha Karmakar, Dorota Grabowska, Gail Sudlow, Kostiantyn Ziabrev, Nibedita Sanyal, and Samuel Achilefu "Effect of linkers on the α_vβ₃ integrin targeting efficiency of cyclic RGD-conjugates", Proc. SPIE 10508, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X, 1050809 (20 February 2018); https://doi.org/10.1117/12.2301223

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