Standards For Corrected Fluorescence Spectra

Ambler Thompson; Kenneth L. Eckerle

doi:10.1117/12.951539

17 May 1989 Standards For Corrected Fluorescence Spectra

Ambler Thompson, Kenneth L. Eckerle

Proceedings Volume 1054, Fluorescence Detection III; (1989) https://doi.org/10.1117/12.951539
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

A set of four fluorescent standards has been produced and calibrated at the National Institute of Standards and Technology (NIST) for corrected relative spectral emission over the wavelength range from 400 to 740 nm. This new Standard Reference Material (SRM) 1931 has been produced in the form of sintered mixtures of inorganic phosphors and polytetrafluorethylene (PTFE) powder for spectral response calibration of spectrofluorimeters used in biology, medicine and studies of molecular structure. They provide a means for correcting spectrofluorimeter errors due to wavelength dependencies of optics, monochromators, and detectors, thus permitting meaningful intercomparison of results obtained in different laboratories.

Citation Download Citation

Ambler Thompson and Kenneth L. Eckerle "Standards For Corrected Fluorescence Spectra", Proc. SPIE 1054, Fluorescence Detection III, (17 May 1989); https://doi.org/10.1117/12.951539

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available